› 2sqc

X-ray diffraction
2Å resolution

SQUALENE-HOPENE CYCLASE FROM ALICYCLOBACILLUS ACIDOCALDARIUS

Released:
DOI: 10.2210/pdb2sqc/pdb
PDB entry 2sqc coloured by chain, front view.
PDB entry 2sqc coloured by chain, side view.
PDB entry 2sqc coloured by chain, top view.
Source organism: Alicyclobacillus acidocaldarius
Primary publication:
The structure of the membrane protein squalene-hopene cyclase at 2.0 A resolution.
Wendt KU, Lenhart A, Schulz GE
J Mol Biol 286 175-87 (1999)
PMID: 9931258

Function and Biology Details

Reactions catalysed: 
Hopan-22-ol = squalene + H(2)O
Squalene = hop-22(29)-ene
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152567 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Squalene--hopene cyclase Chains: A, B
Molecule details ›
Chains: A, B
Length: 631 amino acids
Theoretical weight: 71.62 KDa
Source organism: Alicyclobacillus acidocaldarius
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P33247 (Residues: 1-631; Coverage: 100%)
Gene names: Aaci_2443, shc
Sequence domains:
Structure domains: Glycosyltransferase

Ligands and Environments

1 bound ligand:
Ligand C8E 6 x C8E
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P43212
Unit cell:
a: 118.82Å b: 118.82Å c: 276.2Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.153 0.153 0.187
Expression system: Escherichia coli K-12

Quick links

Citations

27 review citations

Cyclooxygenases: structural, cellular, and molecular biology.
Smith et al. (2000)
26 more

13 mentions without citation

Monotopic Membrane Proteins Join the Fold.
Allen et al. (2019)
12 more