› 2uve

X-ray diffraction
2.19Å resolution

Structure of Yersinia enterocolitica Family 28 Exopolygalacturonase

Released:
DOI: 10.2210/pdb2uve/pdb
PDB entry 2uve coloured by chain, front view.
PDB entry 2uve coloured by chain, side view.
PDB entry 2uve coloured by chain, top view.
Source organism: Yersinia enterocolitica
Primary publication:
The structural basis for exopolygalacturonase activity in a family 28 glycoside hydrolase.
Abbott DW, Boraston AB
J Mol Biol 368 1215-22 (2007)
PMID: 17397864

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130637 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fibronectin type-III domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 608 amino acids
Theoretical weight: 66.73 KDa
Source organism: Yersinia enterocolitica
Expression system: Escherichia coli
UniProt:
  • Canonical: O68975 (Residues: 1-600; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand NI 9 x NI
Ligand SO4 19 x SO4
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS
Spacegroup: P21
Unit cell:
a: 89.952Å b: 78.763Å c: 98.192Å
α: 90° β: 103.61° γ: 90°
R-values:
R R work R free
0.187 0.185 0.228
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Plant Polygalacturonases Involved in Cell Elongation and Separation-The Same but Different?
Babu et al. (2014)
1 more

6 mentions without citation

Structural biology of pectin degradation by Enterobacteriaceae.
Abbott et al. (2008)
5 more