› 2uvo

X-ray diffraction
1.4Å resolution

High Resolution Crystal Structure of Wheat Germ Agglutinin in Complex with N-Acetyl-D-Glucosamine

Released:
DOI: 10.2210/pdb2uvo/pdb
PDB entry 2uvo coloured by chain, front view.
PDB entry 2uvo coloured by chain, side view.
PDB entry 2uvo coloured by chain, top view.
Source organism: Triticum aestivum
Primary publication:
Structural basis of multivalent binding to wheat germ agglutinin.
Schwefel D, Maierhofer C, Beck JG, Seeberger S, Diederichs K, Möller HM, Welte W, Wittmann V
J Am Chem Soc 132 8704-19 (2010)
PMID: 20527753

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145544 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Agglutinin isolectin 1 Chains: A, B, E, F
Molecule details ›
Chains: A, B, E, F
Length: 171 amino acids
Theoretical weight: 17.12 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: P10968 (Residues: 27-197; Coverage: 92%)
Sequence domains: Chitin recognition protein
Structure domains: Endochitinase-like

Ligands and Environments

3 bound ligands:
Ligand NDG 9 x NDG
Ligand NAG 9 x NAG
Ligand GOL 4 x GOL
1 modified residue:
Ligand PCA 4 x PCA

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 44.39Å b: 91.51Å c: 94.5Å
α: 90° β: 82° γ: 90°
R-values:
R R work R free
0.182 0.181 0.204

Quick links

Citations

11 review citations

Bridging lectin binding sites by multivalent carbohydrates.
Wittmann et al. (2013)
10 more

19 mentions without citation

Peptide and protein nanoparticle conjugates: versatile platforms for biomedical applications.
Spicer et al. (2018)
18 more