› 2uw2

X-ray diffraction
2.8Å resolution

Crystal structure of human ribonucleotide reductase subunit R2

Released:
DOI: 10.2210/pdb2uw2/pdb
PDB entry 2uw2 coloured by chain, front view.
PDB entry 2uw2 coloured by chain, side view.
PDB entry 2uw2 coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Welin M, Ogg D, Arrowsmith C, Berglund H, Busam R, Collins R, Edwards A, Ehn M, Flodin S, Flores A, Graslund S, Hammarstrom M, Hallberg BM, Holmberg Schiavone L, Hogbom M, Kotenyova T, Magnusdottir A, Moche M, Nilsson-Ehle P, Nyman T, Persson C, Sagemark J, Sundstrom M, Stenmark P, Uppenberg J, Thorsell AG, Van Den Berg S, Wallden K, Weigelt J, Nordlund P

Function and Biology Details

Reaction catalysed: 
2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H(2)O = ribonucleoside diphosphate + thioredoxin
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151988 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonucleoside-diphosphate reductase subunit M2 Chain: A
Molecule details ›
Chain: A
Length: 332 amino acids
Theoretical weight: 38.69 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P31350 (Residues: 60-389; Coverage: 85%)
Gene names: RR2, RRM2
Sequence domains: Ribonucleotide reductase, small chain
Structure domains: Ribonucleotide Reductase, subunit A

Ligands and Environments

1 bound ligand:
Ligand FE 1 x FE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: C2221
Unit cell:
a: 76.12Å b: 110.7Å c: 94.42Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.233 0.23 0.294
Expression system: Escherichia coli BL21(DE3)

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