› 2uwf

X-ray diffraction
2.1Å resolution

Crystal structure of family 10 xylanase from Bacillus halodurans

Released:
DOI: 10.2210/pdb2uwf/pdb
PDB entry 2uwf coloured by chain, front view.
PDB entry 2uwf coloured by chain, side view.
PDB entry 2uwf coloured by chain, top view.
Source organism: Alkalihalobacillus halodurans
Primary publication:
An alkaline active xylanase: insights into mechanisms of high pH catalytic adaptation.
Mamo G, Thunnissen M, Hatti-Kaul R, Mattiasson B
Biochimie 91 1187-96 (2009)
PMID: 19567261

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139610 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endo-1,4-beta-xylanase A Chain: A
Molecule details ›
Chain: A
Length: 356 amino acids
Theoretical weight: 41.72 KDa
Source organism: Alkalihalobacillus halodurans
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P07528 (Residues: 47-396; Coverage: 95%)
Gene names: BH2120, xynA
Sequence domains: Glycosyl hydrolase family 10
Structure domains: Glycosidases

Ligands and Environments

2 bound ligands:
Ligand CU 1 x CU
Ligand CA 4 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I711
Spacegroup: P212121
Unit cell:
a: 51.602Å b: 53.586Å c: 127.565Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.185 0.241
Expression system: Escherichia coli BL21

Quick links

Citations

2 review citations

Halophiles, coming stars for industrial biotechnology.
Yin et al. (2015)
1 more

8 mentions without citation

Structural Considerations on the Use of Endo-Xylanases for the Production of prebiotic Xylooligosaccharides from Biomass.
Linares-Pasten et al. (2018)
7 more