› 2uxy

X-ray diffraction
1.25Å resolution

Aliphatic amidase

Released:
DOI: 10.2210/pdb2uxy/pdb
PDB entry 2uxy coloured by chain, front view.
PDB entry 2uxy coloured by chain, side view.
PDB entry 2uxy coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Structure of amidase from Pseudomonas aeruginosa showing a trapped acyl transfer reaction intermediate state.
Andrade J, Karmali A, Carrondo MA, Frazão C
J Biol Chem 282 19598-605 (2007)
PMID: 17442671

Function and Biology Details

Reaction catalysed: 
A monocarboxylic acid amide + H(2)O = a monocarboxylate + NH(3)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145824 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aliphatic amidase Chain: A
Molecule details ›
Chain: A
Length: 341 amino acids
Theoretical weight: 38.04 KDa
Source organism: Pseudomonas aeruginosa
UniProt:
  • Canonical: P11436 (Residues: 1-341; Coverage: 99%)
Gene names: PA3366, amiE
Sequence domains: Carbon-nitrogen hydrolase
Structure domains: Carbon-nitrogen hydrolase

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
1 modified residue:
Ligand C3Y 1 x C3Y

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P6322
Unit cell:
a: 102.706Å b: 102.706Å c: 151.32Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.111 not available 0.136

Quick links

Citations

3 review citations

Vaccination against respiratory Pseudomonas aeruginosa infection.
Grimwood et al. (2015)
2 more

7 mentions without citation

A mechanistic view of enzyme evolution.
Yang et al. (2020)
6 more