› 2uz3

X-ray diffraction
2.5Å resolution

Crystal Structure of Thymidine Kinase with dTTP from U. urealyticum

Released:
DOI: 10.2210/pdb2uz3/pdb
PDB entry 2uz3 coloured by chain, front view.
PDB entry 2uz3 coloured by chain, side view.
PDB entry 2uz3 coloured by chain, top view.
Source organism: Ureaplasma urealyticum
Primary publication:
Structures of thymidine kinase 1 of human and mycoplasmic origin.
Welin M, Kosinska U, Mikkelsen NE, Carnrot C, Zhu C, Wang L, Eriksson S, Munch-Petersen B, Eklund H
Proc Natl Acad Sci U S A 101 17970-5 (2004)
PMID: 15611477

Function and Biology Details

Reaction catalysed: 
ATP + thymidine = ADP + thymidine 5'-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192938 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thymidine kinase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 243 amino acids
Theoretical weight: 27.61 KDa
Source organism: Ureaplasma urealyticum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9PPP5 (Residues: 1-223; Coverage: 100%)
Gene names: UU594, tdk
Sequence domains: Thymidine kinase
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand ZN 4 x ZN
Ligand TTP 4 x TTP
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P21
Unit cell:
a: 63.789Å b: 110.753Å c: 73.034Å
α: 90° β: 109.86° γ: 90°
R-values:
R R work R free
0.223 0.22 0.275
Expression system: Escherichia coli BL21(DE3)

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Citations

9 review citations

Single amino acid chelates (SAAC): a strategy for the design of technetium and rhenium radiopharmaceuticals.
Bartholomä et al. (2009)
8 more

3 mentions without citation

3'-(1,2,3-Triazol-1-yl)-3'-deoxythymidine analogs as substrates for human and Ureaplasma parvum thymidine kinase for structure-activity investigations.
Lin et al. (2010)
2 more