› 2v1u

X-ray diffraction
3.1Å resolution

STRUCTURE OF THE AEROPYRUM PERNIX ORC1 PROTEIN IN COMPLEX WITH DNA

Released:
DOI: 10.2210/pdb2v1u/pdb
PDB entry 2v1u coloured by chain, front view.
PDB entry 2v1u coloured by chain, side view.
PDB entry 2v1u coloured by chain, top view.
Source organism: Aeropyrum pernix
Primary publication:
Structural basis of DNA replication origin recognition by an ORC protein.
Gaudier M, Schuwirth BS, Westcott SL, Wigley DB
Science 317 1213-6 (2007)
PMID: 17761880

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115859 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
ORC1-type DNA replication protein 1 Chain: A
Molecule details ›
Chain: A
Length: 387 amino acids
Theoretical weight: 43.5 KDa
Source organism: Aeropyrum pernix
UniProt:
  • Canonical: Q9YEV6 (Residues: 9-395; Coverage: 98%)
Gene names: APE_0475.1, orc1
Sequence domains:
Structure domains:
5'-D(*TP*CP*TP*CP*CP*AP*CP*AP*GP*GP *AP*AP*AP*CP*GP*GP*AP*GP*GP*GP*GP*T)-3' Chain: B
Molecule details ›
Chain: B
Length: 22 nucleotides
Theoretical weight: 6.83 KDa
Source organism: Aeropyrum pernix
Expression system: Not provided
5'-D(*AP*CP*CP*CP*CP*TP*CP*CP*GP*TP *TP*TP*CP*CP*TP*GP*TP*GP*GP*AP*GP*A)-3' Chain: C
Molecule details ›
Chain: C
Length: 22 nucleotides
Theoretical weight: 6.68 KDa
Source organism: Aeropyrum pernix
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand ADP 1 x ADP
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P6522
Unit cell:
a: 75.7Å b: 75.7Å c: 395.11Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.257 0.254 0.297
Expression system: Not provided

Quick links

Citations

35 review citations

The Mcm complex: unwinding the mechanism of a replicative helicase.
Bochman et al. (2009)
34 more

12 mentions without citation

From structure to mechanism-understanding initiation of DNA replication.
Riera et al. (2017)
11 more