› 2v1x

X-ray diffraction
2Å resolution

Crystal structure of human RECQ-like DNA helicase

Released:
DOI: 10.2210/pdb2v1x/pdb
PDB entry 2v1x coloured by chain, front view.
PDB entry 2v1x coloured by chain, side view.
PDB entry 2v1x coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the human RECQ1 helicase reveals a putative strand-separation pin.
Pike AC, Shrestha B, Popuri V, Burgess-Brown N, Muzzolini L, Costantini S, Vindigni A, Gileadi O
Proc Natl Acad Sci U S A 106 1039-44 (2009)
PMID: 19151156

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155416 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATP-dependent DNA helicase Q1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 591 amino acids
Theoretical weight: 67.38 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P46063 (Residues: 49-616; Coverage: 88%)
Gene names: RECQ1, RECQL, RECQL1
Sequence domains:
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand ADP 2 x ADP
Ligand MG 2 x MG
Ligand ZN 2 x ZN
Ligand CL 9 x CL
Ligand EDO 2 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P21
Unit cell:
a: 83.229Å b: 97.647Å c: 85.849Å
α: 90° β: 104.5° γ: 90°
R-values:
R R work R free
0.235 0.232 0.278
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

24 review citations

SF1 and SF2 helicases: family matters.
Fairman-Williams et al. (2010)
23 more

25 mentions without citation

Welcome the family of FANCJ-like helicases to the block of genome stability maintenance proteins.
Wu et al. (2009)
24 more