› 2v4h

X-ray diffraction
2.9Å resolution

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131554 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
NF-kappa-B essential modulator Chains: A, B
Molecule details ›
Chains: A, B
Length: 110 amino acids
Theoretical weight: 12.9 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O88522 (Residues: 251-337; Coverage: 21%)
Gene names: Ikbkg, Nemo
Sequence domains: Leucine zipper of domain CC2 of NEMO, NF-kappa-B essential modulator
Structure domains: Nemo cc2-lz domain - 1d5 darpin complex
1D5 DARPIN Chains: C, D
Molecule details ›
Chains: C, D
Length: 136 amino acids
Theoretical weight: 14.92 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Structure domains: Ankyrin repeat-containing domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P43212
Unit cell:
a: 63.02Å b: 63.02Å c: 436.917Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.208 0.268
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli

Quick links

Citations

9 review citations

Mendelian susceptibility to mycobacterial disease: genetic, immunological, and clinical features of inborn errors of IFN-γ immunity.
Bustamante et al. (2014)
8 more

5 mentions without citation

Structural insights for engineering binding proteins based on non-antibody scaffolds.
Gilbreth et al. (2012)
4 more