› 2v77

X-ray diffraction
1.6Å resolution

Crystal Structure of Human Carboxypeptidase A1

Released:
DOI: 10.2210/pdb2v77/pdb
PDB entry 2v77 coloured by chain, front view.
PDB entry 2v77 coloured by chain, side view.
PDB entry 2v77 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Direct interaction between a human digestive protease and the mucoadhesive poly(acrylic acid).
Pallarès I, Fernández D, Comellas-Bigler M, Fernández-Recio J, Ventura S, Avilés FX, Bode W, Vendrell J
Acta Crystallogr D Biol Crystallogr D64 784-91 (2008)
PMID: 18566513

Function and Biology Details

Reaction catalysed: 
Release of a C-terminal amino acid, but little or no action with -Asp, -Glu, -Arg, -Lys or -Pro.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147208 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carboxypeptidase A1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 309 amino acids
Theoretical weight: 34.7 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P15085 (Residues: 111-419; Coverage: 77%)
Gene names: CPA, CPA1
Sequence domains: Zinc carboxypeptidase
Structure domains: Zn peptidases

Ligands and Environments

3 bound ligands:
Ligand PAY 2 x PAY
Ligand ZN 2 x ZN
Ligand EPE 3 x EPE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MPG/DESY, HAMBURG BEAMLINE BW6
Spacegroup: P43212
Unit cell:
a: 129.565Å b: 129.565Å c: 90.856Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.178 0.192
Expression system: Komagataella pastoris

Quick links

Citations

2 review citations

Progress in metallocarboxypeptidases and their small molecular weight inhibitors.
Fernández et al. (2010)
1 more

2 mentions without citation

The mitosis and neurodevelopment proteins NDE1 and NDEL1 form dimers, tetramers, and polymers with a folded back structure in solution.
Soares et al. (2012)
1 more