› 2v84

X-ray diffraction
1.78Å resolution

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131388 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Spermidine/putrescine ABC transporter, periplasmic binding protein (PotD) Chain: A
Molecule details ›
Chain: A
Length: 343 amino acids
Theoretical weight: 39.14 KDa
Source organism: Treponema pallidum subsp. pallidum str. Nichols
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O83661 (Residues: 25-348; Coverage: 100%)
Gene name: TP_0655
Sequence domains: Bacterial extracellular solute-binding protein
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

2 bound ligands:
Ligand MES 2 x MES
Ligand CL 5 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P212121
Unit cell:
a: 51.61Å b: 86.27Å c: 89.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.236 0.234 0.26
Expression system: Escherichia coli BL21

Quick links

Citations

6 review citations

A multifaceted role for polyamines in bacterial pathogens.
Shah et al. (2008)
5 more

4 mentions without citation

Structural and biochemical basis for polyamine binding to the Tp0655 lipoprotein of Treponema pallidum: putative role for Tp0655 (TpPotD) as a polyamine receptor.
Machius et al. (2007)
3 more