› 2v8g

X-ray diffraction
2.5Å resolution

Crystal structure of beta-alanine synthase from Saccharomyces kluyveri in complex with the product beta-alanine

Released:
DOI: 10.2210/pdb2v8g/pdb
PDB entry 2v8g coloured by chain, front view.
PDB entry 2v8g coloured by chain, side view.
PDB entry 2v8g coloured by chain, top view.
Source organism: Lachancea kluyveri
Primary publication:
Crystal structures of yeast beta-alanine synthase complexes reveal the mode of substrate binding and large scale domain closure movements.
Lundgren S, Andersen B, Piskur J, Dobritzsch D
J Biol Chem 282 36037-47 (2007)
PMID: 17916556

Function and Biology Details

Reaction catalysed: 
3-ureidopropanoate + H(2)O = beta-alanine + CO(2) + NH(3)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188776 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peptidase M20 dimerisation domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 474 amino acids
Theoretical weight: 52.01 KDa
Source organism: Lachancea kluyveri
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q96W94 (Residues: 2-455; Coverage: 100%)
Gene name: PYD3
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand ZN 8 x ZN
Ligand BAL 2 x BAL
Ligand BCN 4 x BCN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P21
Unit cell:
a: 50.1Å b: 217.3Å c: 81.6Å
α: 90° β: 91.9° γ: 90°
R-values:
R R work R free
0.191 0.189 0.231
Expression system: Escherichia coli BL21(DE3)

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Citations

12 citation in other articles

The crystal structure of beta-alanine synthase from Drosophila melanogaster reveals a homooctameric helical turn-like assembly.
Lundgren et al. (2008)
11 more