Function and Biology Details
Reactions catalysed:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate
Biochemical function:
Biological process:
Cellular component:
- not assigned
Sequence domains:
Structure domains:
Structure analysis Details
Assembly composition:
monomeric (preferred)
Assembly name:
DNA polymerase beta (preferred)
PDBe Complex ID:
PDB-CPX-139217 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA polymerase beta
Molecule details ›
Chain: A
Length: 245 amino acids
Theoretical weight: 28.39 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains:
Structure domains:
Length: 245 amino acids
Theoretical weight: 28.39 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical:
P06766 (Residues: 91-335; Coverage: 73%)
Sequence domains:
Structure domains:
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
RIGAKU RU300
Spacegroup:
P21212
Expression system: Escherichia coli BL21(DE3)
