› 2vcp

X-ray diffraction
3.2Å resolution

Crystal structure of N-Wasp VC domain in complex with skeletal actin

Released:
DOI: 10.2210/pdb2vcp/pdb
PDB entry 2vcp coloured by chain, front view.
PDB entry 2vcp coloured by chain, side view.
PDB entry 2vcp coloured by chain, top view.
Source organisms:
Primary publication:
Interactions of isolated C-terminal fragments of neural Wiskott-Aldrich syndrome protein (N-WASP) with actin and Arp2/3 complex.
Gaucher JF, Maugé C, Didry D, Guichard B, Renault L, Carlier MF
J Biol Chem 287 34646-59 (2012)
PMID: 22847007

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126258 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Actin, alpha skeletal muscle Chains: A, B
Molecule details ›
Chains: A, B
Length: 375 amino acids
Theoretical weight: 41.88 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P68135 (Residues: 3-377; Coverage: 100%)
Gene names: ACTA, ACTA1
Sequence domains: Actin
Structure domains:
Actin nucleation-promoting factor WASL Chains: D, E
Molecule details ›
Chains: D, E
Length: 93 amino acids
Theoretical weight: 9.83 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O00401 (Residues: 392-484; Coverage: 18%)
Gene name: WASL
Sequence domains: WH2 motif

Ligands and Environments

2 bound ligands:
Ligand ATP 2 x ATP
Ligand CA 2 x CA
1 modified residue:
Ligand HIC 2 x HIC

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P61
Unit cell:
a: 136.24Å b: 136.24Å c: 205.41Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.275 0.275 0.331
Expression system: Escherichia coli BL21

Quick links

Citations

10 review citations

New insights into the regulation and cellular functions of the ARP2/3 complex.
Rotty et al. (2013)
9 more

8 mentions without citation

Physical mechanisms of signal integration by WASP family proteins.
Padrick et al. (2010)
7 more