› 2vd6

X-ray diffraction
2Å resolution

Human adenylosuccinate lyase in complex with its substrate N6-(1,2- Dicarboxyethyl)-AMP, and its products AMP and fumarate.

Released:
DOI: 10.2210/pdb2vd6/pdb
PDB entry 2vd6 coloured by chain, front view.
PDB entry 2vd6 coloured by chain, side view.
PDB entry 2vd6 coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Stenmark P, Moche M, Arrowsmith C, Berglund H, Busam R, Collins R, Dahlgren LG, Edwards A, Flodin S, Flores A, Graslund S, Hammarstrom M, Hallberg BM, Holmberg-schiavone L, Johansson I, Kallas A, Karlberg T, Kotenyova T, Lehtio L, Nilsson M, Nyman T, Ogg D, Persson C, Sagemark J, Sundstrom M, Thorsell AG, Tresaugues L, van den Berg S, Weigelt J, Welin M, Nordlund P, Structural Genomics Consortium (SGC)

Function and Biology Details

Reaction catalysed: 
(S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate = fumarate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151766 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenylosuccinate lyase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 503 amino acids
Theoretical weight: 57.19 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P30566 (Residues: 1-481; Coverage: 99%)
Gene names: ADSL, AMPS
Sequence domains:
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand AMP 2 x AMP
Ligand FUM 2 x FUM
Ligand GOL 5 x GOL
Ligand CL 7 x CL
Ligand 2SA 2 x 2SA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P212121
Unit cell:
a: 87.3Å b: 128.1Å c: 190.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.192 0.229
Expression system: Escherichia coli BL21(DE3)

Quick links