› 2vda

Solution NMR

Solution structure of the SecA-signal peptide complex

Released:
DOI: 10.2210/pdb2vda/pdb
PDB entry 2vda coloured by chain, ensemble of 10 models, front view.
PDB entry 2vda coloured by chain, ensemble of 10 models, side view.
PDB entry 2vda coloured by chain, ensemble of 10 models, top view.
Source organism: Escherichia coli
Primary publication:
Structural basis for signal-sequence recognition by the translocase motor SecA as determined by NMR.
Gelis I, Bonvin AM, Keramisanou D, Koukaki M, Gouridis G, Karamanou S, Economou A, Kalodimos CG
Cell 131 756-69 (2007)
PMID: 18022369

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + cellular protein(Side 1) = ADP + phosphate + cellular protein(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145294 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein translocase subunit SecA Chain: A
Molecule details ›
Chain: A
Length: 828 amino acids
Theoretical weight: 94 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P10408 (Residues: 9-836; Coverage: 92%)
Gene names: JW0096, azi, b0098, pea, prlD, secA
Sequence domains:
Maltoporin Chain: B
Molecule details ›
Chain: B
Length: 28 amino acids
Theoretical weight: 2.99 KDa
Source organism: Escherichia coli
Expression system: Not provided
UniProt:
  • Canonical: Q8CVI4 (Residues: 1-25; Coverage: 0%)
Gene names: c5006, lamB

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: SEMIRIGID AND FLEXIBLE SIMULATED ANNEALING
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

58 review citations

Using chemical shift perturbation to characterise ligand binding.
Williamson MP. (2013)
57 more

19 mentions without citation

Mechanical design of translocating motor proteins.
Hwang et al. (2009)
18 more