› 2veo

X-ray diffraction
2.2Å resolution

X-ray structure of Candida antarctica lipase A in its closed state.

Released:
DOI: 10.2210/pdb2veo/pdb
PDB entry 2veo coloured by chain, front view.
PDB entry 2veo coloured by chain, side view.
PDB entry 2veo coloured by chain, top view.
Source organism: Moesziomyces antarcticus
Primary publication:
X-ray structure of Candida antarctica lipase A shows a novel lid structure and a likely mode of interfacial activation.
Ericsson DJ, Kasrayan A, Johansson P, Bergfors T, Sandström AG, Bäckvall JE, Mowbray SL
J Mol Biol 376 109-19 (2008)
PMID: 18155238

Function and Biology Details

Reaction catalysed: 
Triacylglycerol + H(2)O = diacylglycerol + a carboxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-197734 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lipase A Chains: A, B
Molecule details ›
Chains: A, B
Length: 441 amino acids
Theoretical weight: 47.2 KDa
Source organism: Moesziomyces antarcticus
Expression system: Komagataella pastoris
UniProt:
  • Canonical: W3VKA4 (Residues: 22-462; Coverage: 100%)
Gene name: PaG_04054
Sequence domains: Secretory lipase
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand IUM 4 x IUM
Ligand PG4 1 x PG4
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P43212
Unit cell:
a: 91.539Å b: 91.539Å c: 299.842Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.19 0.221
Expression system: Komagataella pastoris

Quick links

Citations

12 review citations

Laboratory evolution of stereoselective enzymes: a prolific source of catalysts for asymmetric reactions.
Reetz MT. (2011)
11 more

15 mentions without citation

Combinatorial reshaping of the Candida antarctica lipase A substrate pocket for enantioselectivity using an extremely condensed library.
Sandström et al. (2012)
14 more