› 2vfa

X-ray diffraction
2.8Å resolution

Crystal structure of a chimera of Plasmodium falciparum and human hypoxanthine-guanine phosphoribosyl transferases

Released:
DOI: 10.2210/pdb2vfa/pdb
PDB entry 2vfa coloured by chain, front view.
PDB entry 2vfa coloured by chain, side view.
PDB entry 2vfa coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structure of a chimera of human and Plasmodium falciparum hypoxanthine guanine phosphoribosyltransferases provides insights into oligomerization.
Gayathri P, Sujay Subbayya IN, Ashok CS, Selvi TS, Balaram H, Murthy MR
Proteins 73 1010-20 (2008)
PMID: 18536021

Function and Biology Details

Reactions catalysed: 
XMP + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + xanthine
IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132561 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hypoxanthine-guanine phosphoribosyltransferase; Hypoxanthine-guanine-xanthine phosphoribosyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 229 amino acids
Theoretical weight: 25.83 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: P20035 (Residues: 1-58, 172-231; Coverage: 51%)
  • Canonical: P00492 (Residues: 51-161; Coverage: 51%)
Gene names: HPRT, HPRT1, LACZ
Sequence domains: Phosphoribosyl transferase domain
Structure domains: Rossmann fold

Ligands and Environments

2 bound ligands:
Ligand 5GP 2 x 5GP
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS FR591
Spacegroup: I23
Unit cell:
a: 161.811Å b: 161.811Å c: 161.811Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.233 0.23 0.278
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

Structural and functional studies of the human phosphoribosyltransferase domain containing protein 1.
Welin et al. (2010)
1 more

5 mentions without citation

Inverse docking based screening and identification of protein targets for Cassiarin alkaloids against Plasmodium falciparum.
Negi et al. (2018)
4 more