› 2vgd

X-ray diffraction
1.8Å resolution

Crystal structure of environmental isolated GH11 in complex with xylobiose and feruloyl-arabino-xylotriose

Released:
DOI: 10.2210/pdb2vgd/pdb
PDB entry 2vgd coloured by chain, front view.
PDB entry 2vgd coloured by chain, side view.
PDB entry 2vgd coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Understanding the structural basis for substrate and inhibitor recognition in eukaryotic GH11 xylanases.
Vardakou M, Dumon C, Murray JW, Christakopoulos P, Weiner DP, Juge N, Lewis RJ, Gilbert HJ, Flint JE
J Mol Biol 375 1293-305 (2008)
PMID: 18078955

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-163268 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
ENXYN11A Chain: A
Molecule details ›
Chain: A
Length: 218 amino acids
Theoretical weight: 23.49 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21
Structure domains: Jelly Rolls

Ligands and Environments

Carbohydrate polymer : NEW Components: XYP
Carbohydrate polymer
Carbohydrate polymer : NEW Components: XYP
Carbohydrate polymer
3 bound ligands:
Ligand NA 1 x NA
Ligand FX3 1 x FX3
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P41212
Unit cell:
a: 76.47Å b: 76.47Å c: 102.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.177 0.175 0.203
Expression system: Escherichia coli BL21

Quick links

Citations

5 review citations

Structural determinants of the substrate specificities of xylanases from different glycoside hydrolase families.
Pollet et al. (2010)
4 more

6 mentions without citation

Enzymatic deconstruction of xylan for biofuel production.
Dodd et al. (2009)
5 more