› 2vix

X-ray diffraction
2.85Å resolution

Methylated Shigella flexneri MxiC

Released:
DOI: 10.2210/pdb2vix/pdb
PDB entry 2vix coloured by chain, front view.
PDB entry 2vix coloured by chain, side view.
PDB entry 2vix coloured by chain, top view.
Source organism: Shigella flexneri
Primary publication:
Structures of the Shigella flexneri type 3 secretion system protein MxiC reveal conformational variability amongst homologues.
Deane JE, Roversi P, King C, Johnson S, Lea SM
J Mol Biol 377 985-92 (2008)
PMID: 18304577

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170048 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein MxiC Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 294 amino acids
Theoretical weight: 34.18 KDa
Source organism: Shigella flexneri
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q04640 (Residues: 74-355; Coverage: 79%)
Gene names: CP0146, mxiC
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ACT 1 x ACT
Ligand GOL 2 x GOL
1 modified residue:
Ligand MLY 60 x MLY

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P212121
Unit cell:
a: 88.791Å b: 102.572Å c: 122.638Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.245 0.244 0.273
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

11 review citations

Protein export according to schedule: architecture, assembly, and regulation of type III secretion systems from plant- and animal-pathogenic bacteria.
Büttner D. (2012)
10 more

4 mentions without citation

Structures of the Shigella flexneri type 3 secretion system protein MxiC reveal conformational variability amongst homologues.
Deane et al. (2008)
3 more