› 2vj2

X-ray diffraction
2.5Å resolution

Human Jagged-1, domains DSL and EGFs1-3

Released:
DOI: 10.2210/pdb2vj2/pdb
PDB entry 2vj2 coloured by chain, front view.
PDB entry 2vj2 coloured by chain, side view.
PDB entry 2vj2 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A conserved face of the Jagged/Serrate DSL domain is involved in Notch trans-activation and cis-inhibition.
Cordle J, Johnson S, Tay JZ, Roversi P, Wilkin MB, de Madrid BH, Shimizu H, Jensen S, Whiteman P, Jin B, Redfield C, Baron M, Lea SM, Handford PA
Nat Struct Mol Biol 15 849-57 (2008)
PMID: 18660822

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160269 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein jagged-1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 169 amino acids
Theoretical weight: 18.83 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P78504 (Residues: 185-335; Coverage: 13%)
Gene names: JAG1, JAGL1
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand MLT 1 x MLT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: C2
Unit cell:
a: 61Å b: 102.3Å c: 62.7Å
α: 90° β: 114.3° γ: 90°
R-values:
R R work R free
0.221 0.22 0.239
Expression system: Escherichia coli

Quick links

Citations

38 review citations

The Notch signalling system: recent insights into the complexity of a conserved pathway.
Guruharsha et al. (2012)
37 more

10 mentions without citation

The canonical Notch signaling pathway: unfolding the activation mechanism.
Kopan et al. (2009)
9 more