› 2vui

X-ray diffraction
2.9Å resolution

Crystal structure of the HupR receiver domain in inhibitory phospho- state

Released:
DOI: 10.2210/pdb2vui/pdb
PDB entry 2vui coloured by chain, front view.
PDB entry 2vui coloured by chain, side view.
PDB entry 2vui coloured by chain, top view.
Source organism: Rhodobacter capsulatus
Primary publication:
The HupR receiver domain crystal structure in its nonphospho and inhibitory phospho states.
Davies KM, Lowe ED, Vénien-Bryan C, Johnson LN
J Mol Biol 385 51-64 (2009)
PMID: 18977359

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150740 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hydrogenase transcriptional regulatory protein HupR1 Chain: B
Molecule details ›
Chain: B
Length: 139 amino acids
Theoretical weight: 15.66 KDa
Source organism: Rhodobacter capsulatus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P26408 (Residues: 5-140; Coverage: 28%)
Gene name: hupR1
Sequence domains: Response regulator receiver domain
Structure domains: Rossmann fold

Ligands and Environments

2 bound ligands:
Ligand BEF 1 x BEF
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P3212
Unit cell:
a: 81.893Å b: 81.893Å c: 61.812Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.261 0.258 0.32
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Molecular Mechanisms of Two-Component Signal Transduction.
Zschiedrich et al. (2016)
2 more

2 mentions without citation

FEM: feature-enhanced map.
Afonine et al. (2015)
1 more