› 2vxq

X-ray diffraction
1.9Å resolution

Crystal structure of the major grass pollen allergen Phl p 2 in complex with its specific IgE-Fab

Released:
DOI: 10.2210/pdb2vxq/pdb
PDB entry 2vxq coloured by chain, front view.
PDB entry 2vxq coloured by chain, side view.
PDB entry 2vxq coloured by chain, top view.
Source organisms:
Primary publication:
High-affinity IgE recognition of a conformational epitope of the major respiratory allergen Phl p 2 as revealed by X-ray crystallography.
Padavattan S, Flicker S, Schirmer T, Madritsch C, Randow S, Reese G, Vieths S, Lupinek C, Ebner C, Valenta R, Markovic-Housley Z
J Immunol 182 2141-51 (2009)
PMID: 19201867

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-211222 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Pollen allergen Phl p 2 Chain: A
Molecule details ›
Chain: A
Length: 96 amino acids
Theoretical weight: 10.83 KDa
Source organism: Phleum pratense
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P43214 (Residues: 27-122; Coverage: 100%)
Gene name: PHLPII
Sequence domains: Expansin C-terminal domain
Structure domains: Expansin, cellulose-binding-like domain
FAB Chain: H
Molecule details ›
Chain: H
Length: 216 amino acids
Theoretical weight: 22.98 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
Structure domains: Immunoglobulins
FAB Chain: L
Molecule details ›
Chain: L
Length: 214 amino acids
Theoretical weight: 23.44 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P42212
Unit cell:
a: 105.437Å b: 105.437Å c: 110.225Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.177 0.226
Expression systems:
  • Escherichia coli BL21
  • Cricetulus griseus

Quick links

Citations

17 review citations

From allergen genes to allergy vaccines.
Valenta et al. (2010)
16 more

18 mentions without citation

Relevant B cell epitopes in allergic disease.
Pomés A. (2010)
17 more