› 2vyf

X-ray diffraction
3.6Å resolution

Crystal Structure of the DnaC

Released:
DOI: 10.2210/pdb2vyf/pdb
PDB entry 2vyf coloured by chain, front view.
PDB entry 2vyf coloured by chain, side view.
PDB entry 2vyf coloured by chain, top view.
Source organism: Geobacillus kaustophilus HTA426
Primary publication:
The crystal structure of a replicative hexameric helicase DnaC and its complex with single-stranded DNA.
Lo YH, Tsai KL, Sun YJ, Chen WT, Huang CY, Hsiao CD
Nucleic Acids Res 37 804-14 (2009)
PMID: 19074952

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177353 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Replicative DNA helicase Chains: A, B
Molecule details ›
Chains: A, B
Length: 454 amino acids
Theoretical weight: 50.83 KDa
Source organism: Geobacillus kaustophilus HTA426
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q5KU75 (Residues: 1-454; Coverage: 100%)
Gene name: GK3476
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand AU 4 x AU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: P63
Unit cell:
a: 176.968Å b: 176.968Å c: 108.824Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.293 0.293 0.324
Expression system: Escherichia coli BL21

Quick links

Citations

11 review citations

Mechanisms for initiating cellular DNA replication.
Costa et al. (2013)
10 more

4 mentions without citation

The crystal structure of a replicative hexameric helicase DnaC and its complex with single-stranded DNA.
Lo et al. (2009)
3 more