› 2vz4

X-ray diffraction
2.9Å resolution

The N-terminal domain of MerR-like protein TipAL bound to promoter DNA

Released:
DOI: 10.2210/pdb2vz4/pdb
PDB entry 2vz4 coloured by chain, front view.
PDB entry 2vz4 coloured by chain, side view.
PDB entry 2vz4 coloured by chain, top view.
Source organism: Streptomyces lividans
Entry authors: Allan MG, Stetefeld J, Schirmer T

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115891 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
HTH-type transcriptional activator TipA Chain: A
Molecule details ›
Chain: A
Length: 108 amino acids
Theoretical weight: 12.05 KDa
Source organism: Streptomyces lividans
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A4T9 (Residues: 2-109; Coverage: 43%)
Gene name: tipA
Sequence domains: MerR HTH family regulatory protein
Structure domains: Multidrug-efflux Transporter Regulator; Chain: A; Domain 2
5'-D(*CP*TP*CP*CP*TP*CP*AP*CP*GP*TP *CP*AP*CP*GP*TP*GP*AP*GP*GP*TP*G)-3' Chain: B
Molecule details ›
Chain: B
Length: 26 nucleotides
Theoretical weight: 7.97 KDa
Source organism: Streptomyces lividans
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21212
Unit cell:
a: 45.85Å b: 67.467Å c: 73.129Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.24 0.239 0.274
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

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