› 2w1z

X-ray diffraction
1.97Å resolution

ROP2 from Toxoplasma gondii: A virulence factor with a protein- kinase fold and no enzymatic activity.

Released:
DOI: 10.2210/pdb2w1z/pdb
PDB entry 2w1z coloured by chain, front view.
PDB entry 2w1z coloured by chain, side view.
PDB entry 2w1z coloured by chain, top view.
Source organism: Toxoplasma gondii RH
Primary publication:
ROP2 from Toxoplasma gondii: a virulence factor with a protein-kinase fold and no enzymatic activity.
Labesse G, Gelin M, Bessin Y, Lebrun M, Papoin J, Cerdan R, Arold ST, Dubremetz JF
Structure 17 139-46 (2009)
PMID: 19141290

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173260 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein kinase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 368 amino acids
Theoretical weight: 42.62 KDa
Source organism: Toxoplasma gondii RH
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q27007 (Residues: 195-561; Coverage: 69%)
Sequence domains: Kinase-like
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: C2
Unit cell:
a: 187.609Å b: 51.348Å c: 125.582Å
α: 90° β: 128.65° γ: 90°
R-values:
R R work R free
0.196 0.193 0.24
Expression system: Escherichia coli BL21

Quick links

Citations

13 review citations

Modulation of innate immunity by Toxoplasma gondii virulence effectors.
Hunter et al. (2012)
12 more

10 mentions without citation

Pseudokinases-remnants of evolution or key allosteric regulators?
Zeqiraj et al. (2010)
9 more