› 2w21

X-ray diffraction
2.95Å resolution

Crystal structure of the aminoacid kinase domain of the glutamate 5 kinase of Escherichia coli.

Released:
DOI: 10.2210/pdb2w21/pdb
PDB entry 2w21 coloured by chain, front view.
PDB entry 2w21 coloured by chain, side view.
PDB entry 2w21 coloured by chain, top view.
Source organism: Escherichia coli BL21
Entry authors: Perez-Arellano I, Gil-Ortiz F, Marco-Marin C, Cervera J, Rubio V

Function and Biology Details

Reaction catalysed: 
ATP + L-glutamate = ADP + L-glutamate 5-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141574 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamate 5-kinase Chain: A
Molecule details ›
Chain: A
Length: 259 amino acids
Theoretical weight: 27.28 KDa
Source organism: Escherichia coli BL21
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P0A7B5 (Residues: 1-259; Coverage: 71%)
Gene names: JW0232, b0242, proB
Sequence domains: Amino acid kinase family
Structure domains: Acetylglutamate kinase-like

Ligands and Environments

1 bound ligand:
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: P6222
Unit cell:
a: 141.358Å b: 141.358Å c: 78.373Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.209 0.206 0.259
Expression system: Escherichia coli BL21

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