› 2w40

X-ray diffraction
1.49Å resolution

Crystal structure of Plasmodium falciparum glycerol kinase with bound glycerol

Released:
DOI: 10.2210/pdb2w40/pdb
PDB entry 2w40 coloured by chain, front view.
PDB entry 2w40 coloured by chain, side view.
PDB entry 2w40 coloured by chain, top view.
Source organism: Plasmodium falciparum 3D7
Primary publication:
Structure and non-essential function of glycerol kinase in Plasmodium falciparum blood stages.
Schnick C, Polley SD, Fivelman QL, Ranford-Cartwright LC, Wilkinson SR, Brannigan JA, Wilkinson AJ, Baker DA
Mol Microbiol 71 533-45 (2009)
PMID: 19040641

Function and Biology Details

Reaction catalysed: 
ATP + glycerol = ADP + sn-glycerol 3-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184742 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
glycerol kinase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 503 amino acids
Theoretical weight: 56.86 KDa
Source organism: Plasmodium falciparum 3D7
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q8IDI4 (Residues: 1-501; Coverage: 100%)
Gene name: PF3D7_1351600
Sequence domains:
Structure domains: Nucleotidyltransferase; domain 5

Ligands and Environments

2 bound ligands:
Ligand EDO 62 x EDO
Ligand GOL 12 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P21
Unit cell:
a: 101.915Å b: 100.717Å c: 107.812Å
α: 90° β: 92.56° γ: 90°
R-values:
R R work R free
0.171 0.168 0.21
Expression system: Escherichia coli BL21

Quick links

Citations

2 review citations

Glycerophospholipid acquisition in Plasmodium - a puzzling assembly of biosynthetic pathways.
Déchamps et al. (2010)
1 more

4 mentions without citation

Disordered Protein Kinase Regions in Regulation of Kinase Domain Cores.
Gógl et al. (2019)
3 more