› 2w9m

X-ray diffraction
2.46Å resolution

Structure of family X DNA polymerase from Deinococcus radiodurans

Released:
DOI: 10.2210/pdb2w9m/pdb
PDB entry 2w9m coloured by chain, front view.
PDB entry 2w9m coloured by chain, side view.
PDB entry 2w9m coloured by chain, top view.
Source organism: Deinococcus radiodurans
Primary publication:
The family X DNA polymerase from Deinococcus radiodurans adopts a non-standard extended conformation.
Leulliot N, Cladière L, Lecointe F, Durand D, Hübscher U, van Tilbeurgh H
J Biol Chem 284 11992-9 (2009)
PMID: 19251692

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193328 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA-directed DNA polymerase Chains: A, B
Molecule details ›
Chains: A, B
Length: 578 amino acids
Theoretical weight: 62.2 KDa
Source organism: Deinococcus radiodurans
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9RX48 (Residues: 1-572; Coverage: 100%)
Gene name: DR_0467
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ZN 7 x ZN
Ligand HG 2 x HG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P21
Unit cell:
a: 58.93Å b: 138.25Å c: 67.75Å
α: 90° β: 92.25° γ: 90°
R-values:
R R work R free
0.206 0.203 0.249
Expression system: Escherichia coli

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Citations

4 review citations

Oxidative stress resistance in Deinococcus radiodurans.
Slade et al. (2011)
3 more

8 mentions without citation

Structural features and functional implications of proteins enabling the robustness of Deinococcus radiodurans.
Chen et al. (2020)
7 more