› 2wa6

X-ray diffraction
1.95Å resolution

Structure of the W148R mutant of human filamin b actin binding domain at 1.95 Angstrom resolution

Released:
DOI: 10.2210/pdb2wa6/pdb
PDB entry 2wa6 coloured by chain, front view.
PDB entry 2wa6 coloured by chain, side view.
PDB entry 2wa6 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Disease-associated substitutions in the filamin B actin binding domain confer enhanced actin binding affinity in the absence of major structural disturbance: Insights from the crystal structures of filamin B actin binding domains.
Sawyer GM, Clark AR, Robertson SP, Sutherland-Smith AJ
J Mol Biol 390 1030-47 (2009)
PMID: 19505475

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130920 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Filamin-B Chain: A
Molecule details ›
Chain: A
Length: 245 amino acids
Theoretical weight: 28.11 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O75369 (Residues: 2-242; Coverage: 9%)
Gene names: FLN1L, FLN3, FLNB, TABP, TAP
Sequence domains: Calponin homology (CH) domain
Structure domains: Calponin-like domain

Ligands and Environments

1 bound ligand:
Ligand CO3 2 x CO3
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P212121
Unit cell:
a: 46.031Å b: 69.304Å c: 89.266Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.195 0.234
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Filamins in mechanosensing and signaling.
Razinia et al. (2012)
6 more