› 2wbu

X-ray diffraction
2.5Å resolution

CRYSTAL STRUCTURE OF THE ZINC FINGER DOMAIN OF KLF4 BOUND TO ITS TARGET DNA

Released:
DOI: 10.2210/pdb2wbu/pdb
PDB entry 2wbu coloured by chain, front view.
PDB entry 2wbu coloured by chain, side view.
PDB entry 2wbu coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
The structure of the Klf4 DNA-binding domain links to self-renewal and macrophage differentiation.
Schuetz A, Nana D, Rose C, Zocher G, Milanovic M, Koenigsmann J, Blasig R, Heinemann U, Carstanjen D
Cell Mol Life Sci 68 3121-31 (2011)
PMID: 21290164

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115906 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Krueppel-like factor 4 Chain: A
Molecule details ›
Chain: A
Length: 90 amino acids
Theoretical weight: 10.57 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q60793 (Residues: 395-483; Coverage: 18%)
Gene names: Ezf, Gklf, Klf4, Zie
Sequence domains: Zinc finger, C2H2 type
Structure domains: Classic Zinc Finger
5'-D(*DGP*DAP*DGP*DGP*DCP*DGP*DTP* DGP*DGP*DC)-3' Chain: B
Molecule details ›
Chain: B
Length: 10 nucleotides
Theoretical weight: 3.13 KDa
Source organism: Mus musculus
Expression system: Not provided
5'-D(*DGP*DCP*DCP*DAP*DCP*DGP*DCP* DCP*DTP*DC)-3' Chain: C
Molecule details ›
Chain: C
Length: 10 nucleotides
Theoretical weight: 2.97 KDa
Source organism: Mus musculus
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 3 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P43212
Unit cell:
a: 50.275Å b: 50.275Å c: 131.027Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.214 0.278
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

10 review citations

Advances in meningioma genetics: novel therapeutic opportunities.
Preusser et al. (2018)
9 more

16 mentions without citation

Evolving insights on how cytosine methylation affects protein-DNA binding.
Dantas Machado et al. (2015)
15 more