› 2wqx

X-ray diffraction
2.03Å resolution

InlB321_4R: S199R, D200R, G206R, A227R, C242A mutant of the Listeria monocytogenes InlB internalin domain

Released:
DOI: 10.2210/pdb2wqx/pdb
PDB entry 2wqx coloured by chain, front view.
PDB entry 2wqx coloured by chain, side view.
PDB entry 2wqx coloured by chain, top view.
Source organism: Listeria monocytogenes EGD-e
Primary publication:
Ligand-mediated dimerization of the Met receptor tyrosine kinase by the bacterial invasion protein InlB.
Ferraris DM, Gherardi E, Di Y, Heinz DW, Niemann HH
J Mol Biol 395 522-32 (2010)
PMID: 19900460

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-144108 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Internalin B Chains: A, B
Molecule details ›
Chains: A, B
Length: 289 amino acids
Theoretical weight: 32.51 KDa
Source organism: Listeria monocytogenes EGD-e
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0DQD2 (Residues: 36-321; Coverage: 48%)
Gene names: inlB, lmo0434
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P1
Unit cell:
a: 38.7Å b: 57.45Å c: 64.3Å
α: 102.99° β: 90.21° γ: 93.56°
R-values:
R R work R free
0.197 0.194 0.257
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

Targeting MET in cancer: rationale and progress.
Gherardi et al. (2012)
8 more