› 2wrw

X-ray diffraction
2.41Å resolution

Semi-synthetic highly active analogue of human insulin D-ProB26-DTI- NH2

Released:
DOI: 10.2210/pdb2wrw/pdb
PDB entry 2wrw coloured by chain, front view.
PDB entry 2wrw coloured by chain, side view.
PDB entry 2wrw coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Implications for the active form of human insulin based on the structural convergence of highly active hormone analogues.
Jirácek J, Záková L, Antolíková E, Watson CJ, Turkenburg JP, Dodson GG, Brzozowski AM
Proc Natl Acad Sci U S A 107 1966-70 (2010)
PMID: 20133841

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134210 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Insulin A chain Chain: A
Molecule details ›
Chain: A
Length: 21 amino acids
Theoretical weight: 2.38 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P01308 (Residues: 90-110; Coverage: 24%)
Gene name: INS
Insulin B chain Chain: B
Molecule details ›
Chain: B
Length: 26 amino acids
Theoretical weight: 2.94 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P01308 (Residues: 25-50; Coverage: 30%)
Gene name: INS

Ligands and Environments

No bound ligands
1 modified residue:
Ligand PR9 1 x PR9

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: I4122
Unit cell:
a: 39.372Å b: 39.372Å c: 123.928Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.239 0.237 0.301
Expression system: Not provided

Quick links

Citations

4 review citations

Insulin receptor and cancer.
Belfiore et al. (2011)
3 more

1 mention without citation

How insulin engages its primary binding site on the insulin receptor.
Menting et al. (2013)