› 2wse

X-ray diffraction
3.49Å resolution

Improved Model of Plant Photosystem I

Released:
DOI: 10.2210/pdb2wse/pdb
PDB entry 2wse coloured by chain, front view.
PDB entry 2wse coloured by chain, side view.
PDB entry 2wse coloured by chain, top view.
Source organisms:
Primary publication:
Structure determination and improved model of plant photosystem I.
Amunts A, Toporik H, Borovikova A, Nelson N
J Biol Chem 285 3478-86 (2010)
PMID: 19923216

Function and Biology Details

Reaction catalysed: 
Reduced plastocyanin + oxidized ferredoxin + light = oxidized plastocyanin + reduced ferredoxin
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octadecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111633 (preferred)
Entry contents:
18 distinct polypeptide molecules
Macromolecules (19 distinct):
Chlorophyll a-b binding protein 6, chloroplastic Chain: 1
Molecule details ›
Chain: 1
Length: 241 amino acids
Theoretical weight: 26.02 KDa
Source organism: Arabidopsis thaliana
UniProt:
  • Canonical: Q01667 (Residues: 1-241; Coverage: 100%)
Gene names: At3g54890, CAB6, F28P10.130, LHCA1
Sequence domains: Chlorophyll A-B binding protein
Chlorophyll a-b binding protein, chloroplastic Chain: 2
Molecule details ›
Chain: 2
Length: 269 amino acids
Theoretical weight: 28.93 KDa
Source organism: Pisum sativum
UniProt:
  • Canonical: Q41038 (Residues: 1-252, 253-269; Coverage: 100%)
Gene names: KIW84_045602, lhaB
Sequence domains: Chlorophyll A-B binding protein
Chlorophyll a-b binding protein, chloroplastic Chain: 3
Molecule details ›
Chain: 3
Length: 276 amino acids
Theoretical weight: 29.85 KDa
Source organism: Glycine max
UniProt:
  • Canonical: C6TEX2 (Residues: 1-276; Coverage: 100%)
Sequence domains: Chlorophyll A-B binding protein
Chlorophyll a-b binding protein P4, chloroplastic Chain: 4
Molecule details ›
Chain: 4
Length: 251 amino acids
Theoretical weight: 27.18 KDa
Source organism: Pisum sativum
UniProt:
  • Canonical: Q9SQL2 (Residues: 1-252; Coverage: 100%)
Gene name: lhcA-P4
Sequence domains: Chlorophyll A-B binding protein
Photosystem I P700 chlorophyll a apoprotein A1 Chain: A
Molecule details ›
Chain: A
Length: 758 amino acids
Theoretical weight: 84.28 KDa
Source organism: Pisum sativum
UniProt:
  • Canonical: P05310 (Residues: 1-758; Coverage: 100%)
Gene names: psaA, psaA1
Sequence domains: Photosystem I psaA/psaB protein
Structure domains: Photosystem I PsaA/PsaB
Photosystem I P700 chlorophyll a apoprotein A2 Chain: B
Molecule details ›
Chain: B
Length: 734 amino acids
Theoretical weight: 82.49 KDa
Source organism: Pisum sativum
UniProt:
  • Canonical: P05311 (Residues: 1-734; Coverage: 100%)
Gene names: psaA2, psaB
Sequence domains: Photosystem I psaA/psaB protein
Structure domains: Photosystem I PsaA/PsaB
Photosystem I iron-sulfur center Chain: C
Molecule details ›
Chain: C
Length: 81 amino acids
Theoretical weight: 8.99 KDa
Source organism: Pisum sativum
UniProt:
  • Canonical: P10793 (Residues: 1-81; Coverage: 100%)
Gene names: frxA, psaC
Sequence domains: 4Fe-4S dicluster domain
Structure domains: Alpha-Beta Plaits
Photosystem I reaction center subunit II, chloroplastic Chain: D
Molecule details ›
Chain: D
Length: 212 amino acids
Theoretical weight: 23.17 KDa
Source organism: Spinacia oleracea
UniProt:
  • Canonical: P12353 (Residues: 1-212; Coverage: 100%)
Gene name: psaD
Sequence domains: PsaD
Structure domains: Photosystem I PsaD, reaction center subunit II
Photosystem I reaction center subunit IV A, chloroplastic Chain: E
Molecule details ›
Chain: E
Length: 143 amino acids
Theoretical weight: 14.98 KDa
Source organism: Arabidopsis thaliana
UniProt:
  • Canonical: Q9S831 (Residues: 1-143; Coverage: 100%)
Gene names: At4g28750, F16A16.140, PSAE1
Sequence domains: Photosystem I reaction centre subunit IV / PsaE
Structure domains: SH3 type barrels.
Photosystem I reaction center subunit III, chloroplastic Chain: F
Molecule details ›
Chain: F
Length: 231 amino acids
Theoretical weight: 25.45 KDa
Source organism: Spinacia oleracea
UniProt:
  • Canonical: P12355 (Residues: 1-231; Coverage: 100%)
Gene name: PSAF
Sequence domains: Photosystem I reaction centre subunit III
Structure domains: Photosystem I PsaF, reaction centre subunit III
Photosystem I reaction center subunit V, chloroplastic Chain: G
Molecule details ›
Chain: G
Length: 167 amino acids
Theoretical weight: 18.23 KDa
Source organism: Spinacia oleracea
UniProt:
  • Canonical: P12357 (Residues: 1-167; Coverage: 100%)
Gene name: PSAG
Sequence domains: Photosystem I psaG / psaK
Structure domains: Chlorophyll a-b binding protein like
Photosystem I reaction center subunit VI, chloroplastic Chain: H
Molecule details ›
Chain: H
Length: 144 amino acids
Theoretical weight: 15.34 KDa
Source organism: Spinacia oleracea
UniProt:
  • Canonical: P22179 (Residues: 1-144; Coverage: 100%)
Gene name: PSAH
Sequence domains: Photosystem I reaction centre subunit VI
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Photosystem I reaction center subunit VIII Chain: I
Molecule details ›
Chain: I
Length: 40 amino acids
Theoretical weight: 4.47 KDa
Source organism: Pisum sativum
UniProt:
  • Canonical: P17227 (Residues: 1-40; Coverage: 100%)
Gene name: psaI
Sequence domains: Photosystem I reaction centre subunit VIII
Photosystem I reaction center subunit IX Chain: J
Molecule details ›
Chain: J
Length: 44 amino acids
Theoretical weight: 5.08 KDa
Source organism: Spinacia oleracea
UniProt:
  • Canonical: P17230 (Residues: 1-44; Coverage: 100%)
Gene name: psaJ
Sequence domains: Photosystem I reaction centre subunit IX / PsaJ
Structure domains: Single helix bin
Photosystem I reaction center subunit psaK, chloroplastic Chain: K
Molecule details ›
Chain: K
Length: 131 amino acids
Theoretical weight: 13.74 KDa
Source organism: Hordeum vulgare
UniProt:
  • Canonical: P36886 (Residues: 1-131; Coverage: 100%)
Gene name: PSAK
Sequence domains: Photosystem I psaG / psaK
Structure domains: Chlorophyll a-b binding protein like
Photosystem I reaction center subunit XI, chloroplastic Chain: L
Molecule details ›
Chain: L
Length: 216 amino acids
Theoretical weight: 22.96 KDa
Source organism: Spinacia oleracea
UniProt:
  • Canonical: Q41385 (Residues: 1-216; Coverage: 100%)
Gene name: PSAL
Sequence domains: Photosystem I reaction centre subunit XI
Structure domains: Photosystem I PsaL, reaction centre subunit XI
Photosystem I-N subunit Chain: N
Molecule details ›
Chain: N
Length: 170 amino acids
Theoretical weight: 18.46 KDa
Source organism: Phaseolus vulgaris
UniProt:
  • Canonical: Q84U30 (Residues: 1-170; Coverage: 100%)
Gene names: PHAVU_002G269400g, PSI-N
Sequence domains: Photosystem I reaction centre subunit N (PSAN or PSI-N)
Structure domains: Chlorophyll A-B binding protein
PHOTOSYSTEM I-N SUBUNIT Chain: R
Molecule details ›
Chain: R
Length: 53 amino acids
Theoretical weight: 4.53 KDa
Source organism: Pisum sativum

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC, FRU
Carbohydrate polymer
7 bound ligands:
Ligand CLA 173 x CLA
Ligand LMU 51 x LMU
Ligand BCR 18 x BCR
Ligand PQN 2 x PQN
Ligand SF4 3 x SF4
Ligand LMG 1 x LMG
Ligand UNL 1 x UNL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P21
Unit cell:
a: 120.655Å b: 189.086Å c: 129.388Å
α: 90° β: 91.24° γ: 90°
R-values:
R R work R free
0.369 0.369 0.375

Quick links

Citations

27 review citations

Evolution of photosynthesis.
Hohmann-Marriott et al. (2011)
26 more

2 mentions without citation

Structure determination and improved model of plant photosystem I.
Amunts et al. (2010)
1 more