› 2wvi

X-ray diffraction
1.8Å resolution

Crystal Structure of the N-terminal Domain of BubR1

Released:
DOI: 10.2210/pdb2wvi/pdb
PDB entry 2wvi coloured by chain, front view.
PDB entry 2wvi coloured by chain, side view.
PDB entry 2wvi coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Defining the molecular basis of BubR1 kinetochore interactions and APC/C-CDC20 inhibition.
D'Arcy S, Davies OR, Blundell TL, Bolanos-Garcia VM
J Biol Chem 285 14764-76 (2010)
PMID: 20220147

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130027 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mitotic checkpoint serine/threonine-protein kinase BUB1 beta Chain: A
Molecule details ›
Chain: A
Length: 164 amino acids
Theoretical weight: 19.6 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O60566 (Residues: 57-220; Coverage: 16%)
Gene names: BUB1B, BUBR1, MAD3L, SSK1
Sequence domains: Mad3/BUB1 homology region 1
Structure domains: Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat

Ligands and Environments

3 bound ligands:
Ligand PR 2 x PR
Ligand ACT 1 x ACT
Ligand EDO 2 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P3221
Unit cell:
a: 62.796Å b: 62.796Å c: 90.45Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.224 0.222 0.26
Expression system: Escherichia coli BL21(DE3)

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Citations

4 review citations

Signalling dynamics in the spindle checkpoint response.
London et al. (2014)
3 more

10 mentions without citation

BUB1 and BUBR1: multifaceted kinases of the cell cycle.
Bolanos-Garcia et al. (2011)
9 more