› 2wvj

X-ray diffraction
2.2Å resolution

Mutation of Thr163 to Ser in Human Thymidine Kinase Shifts the Specificity from Thymidine towards the Nucleoside Analogue Azidothymidine

Released:
DOI: 10.2210/pdb2wvj/pdb
PDB entry 2wvj coloured by chain, front view.
PDB entry 2wvj coloured by chain, side view.
PDB entry 2wvj coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Comparative active-site mutation study of human and Caenorhabditis elegans thymidine kinase 1.
Skovgaard T, Uhlin U, Munch-Petersen B
FEBS J 279 1777-87 (2012)
PMID: 22385435

Function and Biology Details

Reaction catalysed: 
ATP + thymidine = ADP + thymidine 5'-phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137495 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thymidine kinase, cytosolic Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 193 amino acids
Theoretical weight: 21.31 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P04183 (Residues: 1-193; Coverage: 83%)
Gene name: TK1
Sequence domains: Thymidine kinase
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand TTP 8 x TTP
Ligand ZN 8 x ZN
Ligand MG 3 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: C2
Unit cell:
a: 156.902Å b: 123.252Å c: 121.024Å
α: 90° β: 132.98° γ: 90°
R-values:
R R work R free
0.176 0.173 0.221
Expression system: Escherichia coli BL21

Quick links

Citations

1 review citation

Thymidine kinase 1 through the ages: a comprehensive review.
Bitter et al. (2020)
3 other articles

2 mentions without citation

Unusual arginine formations in protein function and assembly: rings, strings, and stacks.
Neves et al. (2012)
1 more