› 2x06

X-ray diffraction
2.5Å resolution

SULFOLACTATE DEHYDROGENASE FROM METHANOCALDOCOCCUS JANNASCHII

Released:
DOI: 10.2210/pdb2x06/pdb
PDB entry 2x06 coloured by chain, front view.
PDB entry 2x06 coloured by chain, side view.
PDB entry 2x06 coloured by chain, top view.
Source organism: Methanocaldococcus jannaschii
Primary publication:
Methanoarchaeal sulfolactate dehydrogenase: prototype of a new family of NADH-dependent enzymes.
Irimia A, Madern D, Zaccaï G, Vellieux FM
EMBO J 23 1234-44 (2004)
PMID: 15014443

Function and Biology Details

Reaction catalysed: 
A (2S)-2-hydroxycarboxylate + NAD(+) = a 2-oxocarboxylate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176696 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-sulfolactate dehydrogenase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 344 amino acids
Theoretical weight: 37.83 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q58820 (Residues: 1-344; Coverage: 100%)
Gene names: MJ1425, comC, mdh
Sequence domains: Malate/L-lactate dehydrogenase
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 8 x NAD
No bound ligands
1 modified residue:
Ligand MSE 72 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: P21
Unit cell:
a: 80.93Å b: 203.71Å c: 100.05Å
α: 90° β: 112.05° γ: 90°
R-values:
R R work R free
0.157 0.153 0.224
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

A new family of NAD(P)H-dependent oxidoreductases distinct from conventional Rossmann-fold proteins.
Muramatsu et al. (2005)
7 other articles

2 mentions without citation

Mechanisms of DNA Transposition.
Hickman et al. (2015)
1 more