› 2x12

X-ray diffraction
2.9Å resolution

pH-induced modulation of Streptococcus parasanguinis adhesion by Fap1 fimbriae

Released:
DOI: 10.2210/pdb2x12/pdb
PDB entry 2x12 coloured by chain, front view.
PDB entry 2x12 coloured by chain, side view.
PDB entry 2x12 coloured by chain, top view.
Source organism: Streptococcus parasanguinis
Primary publication:
Structural insights into serine-rich fimbriae from Gram-positive bacteria.
Ramboarina S, Garnett JA, Zhou M, Li Y, Peng Z, Taylor JD, Lee WC, Bodey A, Murray JW, Alguel Y, Bergeron J, Bardiaux B, Sawyer E, Isaacson R, Tagliaferri C, Cota E, Nilges M, Simpson P, Ruiz T, Wu H, Matthews S
J Biol Chem 285 32446-57 (2010)
PMID: 20584910

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106579 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fap1 adhesin Chains: A, B
Molecule details ›
Chains: A, B
Length: 348 amino acids
Theoretical weight: 37.26 KDa
Source organism: Streptococcus parasanguinis
Expression system: Escherichia coli
UniProt:
  • Canonical: A1C3L3 (Residues: 190-522; Coverage: 13%)
Gene name: fap1
Structure domains: Immunoglobulin-like

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 10 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P3221
Unit cell:
a: 108.668Å b: 108.668Å c: 126.252Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.213 0.211 0.25
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Microbial interactions in building of communities.
Wright et al. (2013)
8 more

7 mentions without citation

Interactions in bacterial biofilm development: a structural perspective.
Garnett et al. (2012)
6 more