› 2x19

X-ray diffraction
2.8Å resolution

Crystal structure of Importin13 - RanGTP complex

Released:
DOI: 10.2210/pdb2x19/pdb
PDB entry 2x19 coloured by chain, front view.
PDB entry 2x19 coloured by chain, side view.
PDB entry 2x19 coloured by chain, top view.
Source organisms:
Primary publication:
Nuclear import mechanism of the EJC component Mago-Y14 revealed by structural studies of importin 13.
Bono F, Cook AG, Grünwald M, Ebert J, Conti E
Mol Cell 37 211-22 (2010)
PMID: 20122403

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131769 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
GTP-binding nuclear protein GSP1/CNR1 Chain: A
Molecule details ›
Chain: A
Length: 172 amino acids
Theoretical weight: 19.77 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P32835 (Residues: 8-179; Coverage: 79%)
Gene names: CNR1, CST17, GSP1, L8003.19, YLR293C
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Importin-13 Chain: B
Molecule details ›
Chain: B
Length: 963 amino acids
Theoretical weight: 109.37 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O94829 (Residues: 1-963; Coverage: 100%)
Gene names: IPO13, KIAA0724, RANBP13
Sequence domains:
Structure domains: Leucine-rich Repeat Variant

Ligands and Environments

2 bound ligands:
Ligand GTP 1 x GTP
Ligand MG 1 x MG
1 modified residue:
Ligand MSE 23 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P3121
Unit cell:
a: 99.93Å b: 99.93Å c: 276.84Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.236 0.236 0.268
Expression system: Escherichia coli BL21

Quick links

Citations

13 review citations

Nuclear import by karyopherin-βs: recognition and inhibition.
Chook et al. (2011)
12 more

8 mentions without citation

Karyopherin-mediated nucleocytoplasmic transport.
Wing et al. (2022)
7 more