› 2x3t

X-ray diffraction
2.75Å resolution

Glutaraldehyde-crosslinked wheat germ agglutinin isolectin 1 crystal soaked with a synthetic glycopeptide

Released:
DOI: 10.2210/pdb2x3t/pdb
PDB entry 2x3t coloured by chain, front view.
PDB entry 2x3t coloured by chain, side view.
PDB entry 2x3t coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of multivalent binding to wheat germ agglutinin.
Schwefel D, Maierhofer C, Beck JG, Seeberger S, Diederichs K, Möller HM, Welte W, Wittmann V
J Am Chem Soc 132 8704-19 (2010)
PMID: 20527753

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145546 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Agglutinin isolectin 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 171 amino acids
Theoretical weight: 17.12 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: P10968 (Residues: 27-197; Coverage: 92%)
Sequence domains: Chitin recognition protein
Structure domains: Endochitinase-like
GLYCOPEPTIDE Chains: E, M
Molecule details ›
Chains: E, M
Length: 9 amino acids
Theoretical weight: 886 Da
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand GYV 11 x GYV
Ligand DBB 7 x DBB
Ligand GOL 3 x GOL
3 modified residues:
Ligand PCA 4 x PCA
Ligand DBB 8 x DBB
Ligand DVA 4 x DVA

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P21
Unit cell:
a: 44.64Å b: 93.21Å c: 92.5Å
α: 90° β: 98.32° γ: 90°
R-values:
R R work R free
0.232 0.228 0.285
Expression system: Not provided

Quick links

Citations

14 review citations

Bridging lectin binding sites by multivalent carbohydrates.
Wittmann et al. (2013)
13 more

2 mentions without citation

Structure of allergens and structure based epitope predictions.
Dall'antonia et al. (2014)
1 more