› 2x4w

X-ray diffraction
1.5Å resolution

Molecular basis of Histone H3K36me3 recognition by the PWWP domain of BRPF1.

Released:
DOI: 10.2210/pdb2x4w/pdb
PDB entry 2x4w coloured by chain, front view.
PDB entry 2x4w coloured by chain, side view.
PDB entry 2x4w coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular basis of histone H3K36me3 recognition by the PWWP domain of Brpf1.
Vezzoli A, Bonadies N, Allen MD, Freund SM, Santiveri CM, Kvinlaug BT, Huntly BJ, Göttgens B, Bycroft M
Nat Struct Mol Biol 17 617-9 (2010)
PMID: 20400950

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157224 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Peregrin Chain: A
Molecule details ›
Chain: A
Length: 132 amino acids
Theoretical weight: 15.25 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P55201 (Residues: 1076-1205; Coverage: 11%)
Gene names: BR140, BRPF1
Sequence domains: PWWP domain
Structure domains: SH3 type barrels.
Histone H3.2 Chain: B
Molecule details ›
Chain: B
Length: 21 amino acids
Theoretical weight: 2.33 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q71DI3 (Residues: 23-43; Coverage: 15%)
Gene names: H3C13, H3C14, H3C15, H3F2, H3FM, HIST2H3A, HIST2H3C, HIST2H3D

Ligands and Environments

1 bound ligand:
Ligand FMT 1 x FMT
1 modified residue:
Ligand M3L 1 x M3L

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: I222
Unit cell:
a: 43.261Å b: 71.053Å c: 113.982Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.189 0.216
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

39 review citations

Understanding the language of Lys36 methylation at histone H3.
Wagner et al. (2012)
38 more

8 mentions without citation

Readout of epigenetic modifications.
Patel et al. (2013)
7 more