› 2x8g

X-ray diffraction
1.9Å resolution

Oxidized thioredoxin glutathione reductase from Schistosoma mansoni

Released:
DOI: 10.2210/pdb2x8g/pdb
PDB entry 2x8g coloured by chain, front view.
PDB entry 2x8g coloured by chain, side view.
PDB entry 2x8g coloured by chain, top view.
Source organism: Schistosoma mansoni
Primary publication:
Mapping the catalytic cycle of Schistosoma mansoni thioredoxin glutathione reductase by X-ray crystallography.
Angelucci F, Dimastrogiovanni D, Boumis G, Brunori M, Miele AE, Saccoccia F, Bellelli A
J Biol Chem 285 32557-67 (2010)
PMID: 20659890

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188301 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
thioredoxin-disulfide reductase Chain: A
Molecule details ›
Chain: A
Length: 598 amino acids
Theoretical weight: 65.06 KDa
Source organism: Schistosoma mansoni
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q962Y6 (Residues: 1-598; Coverage: 100%)
Gene name: TGR
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand FAD 1 x FAD
Ligand PEG 4 x PEG
Ligand GOL 2 x GOL
Ligand PG4 1 x PG4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY
Spacegroup: C2
Unit cell:
a: 141.595Å b: 102.785Å c: 59.216Å
α: 90° β: 112.39° γ: 90°
R-values:
R R work R free
0.19 0.189 0.21
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Schistosomiasis control: praziquantel forever?
Cioli et al. (2014)
5 more

4 mentions without citation

Mapping the catalytic cycle of Schistosoma mansoni thioredoxin glutathione reductase by X-ray crystallography.
Angelucci et al. (2010)
3 more