› 2xbq

X-ray diffraction
1.67Å resolution

Crystal structure of reduced Schistosoma mansoni Thioredoxin pre- protein at 1.7 Angstrom

Released:
DOI: 10.2210/pdb2xbq/pdb
PDB entry 2xbq coloured by chain, front view.
PDB entry 2xbq coloured by chain, side view.
PDB entry 2xbq coloured by chain, top view.
Source organism: Schistosoma mansoni
Primary publication:
Structural and functional characterization of Schistosoma mansoni Thioredoxin.
Boumis G, Angelucci F, Bellelli A, Brunori M, Dimastrogiovanni D, Miele AE
Protein Sci 20 1069-76 (2011)
PMID: 21465612

Function and Biology Details

Reaction catalysed: 
Protein dithiol + NAD(P)(+) = protein disulfide + NAD(P)H
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186151 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thioredoxin domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 117 amino acids
Theoretical weight: 13.1 KDa
Source organism: Schistosoma mansoni
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8T9N5 (Residues: 1-106; Coverage: 100%)
Sequence domains: Thioredoxin
Structure domains: Glutaredoxin

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P32
Unit cell:
a: 62.143Å b: 62.143Å c: 58.292Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.216 0.215 0.242
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Thioredoxin glutathione reductase-dependent redox networks in platyhelminth parasites.
Williams et al. (2013)
4 more

2 mentions without citation

Mapping the catalytic cycle of Schistosoma mansoni thioredoxin glutathione reductase by X-ray crystallography.
Angelucci et al. (2010)
1 more