› 2xe6

X-ray diffraction
1.74Å resolution

The complete reaction cycle of human phosphoglycerate kinase: The open binary complex with 3PG

Released:
DOI: 10.2210/pdb2xe6/pdb
PDB entry 2xe6 coloured by chain, front view.
PDB entry 2xe6 coloured by chain, side view.
PDB entry 2xe6 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A spring-loaded release mechanism regulates domain movement and catalysis in phosphoglycerate kinase.
Zerrad L, Merli A, Schröder GF, Varga A, Gráczer É, Pernot P, Round A, Vas M, Bowler MW
J Biol Chem 286 14040-8 (2011)
PMID: 21349853

Function and Biology Details

Reaction catalysed: 
ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132717 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoglycerate kinase 1 Chain: A
Molecule details ›
Chain: A
Length: 417 amino acids
Theoretical weight: 44.67 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P00558 (Residues: 1-417; Coverage: 100%)
Gene names: MIG10, OK/SW-cl.110, PGK1, PGKA
Sequence domains: Phosphoglycerate kinase
Structure domains: Phosphoglycerate kinase, N-terminal domain

Ligands and Environments

1 bound ligand:
Ligand 3PG 1 x 3PG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P21212
Unit cell:
a: 61.57Å b: 73.01Å c: 93.64Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.206 0.252
Expression system: Escherichia coli BL21

Quick links

Citations

3 review citations

Carbohydrate metabolism in Archaea: current insights into unusual enzymes and pathways and their regulation.
Bräsen et al. (2014)
2 more

2 mentions without citation

Terazosin activates Pgk1 and Hsp90 to promote stress resistance.
Chen et al. (2015)
1 more