› 2xmn

X-ray diffraction
2.85Å resolution

High resolution snapshots of defined TolC open states present an iris- like movement of periplasmic entrance helices

Released:
DOI: 10.2210/pdb2xmn/pdb
PDB entry 2xmn coloured by chain, front view.
PDB entry 2xmn coloured by chain, side view.
PDB entry 2xmn coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Structures of sequential open states in a symmetrical opening transition of the TolC exit duct.
Pei XY, Hinchliffe P, Symmons MF, Koronakis E, Benz R, Hughes C, Koronakis V
Proc Natl Acad Sci U S A 108 2112-7 (2011)
PMID: 21245342

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136391 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Outer membrane protein TolC Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 428 amino acids
Theoretical weight: 46.92 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P02930 (Residues: 23-450; Coverage: 91%)
Gene names: JW5503, b3035, colE1-i, mtcB, mukA, refI, toc, tolC, weeA
Sequence domains: Outer membrane efflux protein
Structure domains: Outer membrane efflux proteins (OEP)

Ligands and Environments

2 bound ligands:
Ligand CL 3 x CL
Ligand LMT 1 x LMT
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 134.124Å b: 135.524Å c: 136.343Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.273 0.271 0.309
Expression system: Escherichia coli

Quick links

Citations

19 review citations

Secretion systems in Gram-negative bacteria: structural and mechanistic insights.
Costa et al. (2015)
18 more

8 mentions without citation

The bacterial outer membrane β-barrel assembly machinery.
Kim et al. (2012)
7 more