› 2xn9

X-ray diffraction
2.3Å resolution

Crystal structure of the ternary complex between human T cell receptor, staphylococcal enterotoxin H and human major histocompatibility complex class II

Released:
DOI: 10.2210/pdb2xn9/pdb
PDB entry 2xn9 coloured by chain, front view.
PDB entry 2xn9 coloured by chain, side view.
PDB entry 2xn9 coloured by chain, top view.
Source organisms:
Primary publication:
The structure of superantigen complexed with TCR and MHC reveals novel insights into superantigenic T cell activation.
Saline M, Rödström KE, Fischer G, Orekhov VY, Karlsson BG, Lindkvist-Petersson K
Nat Commun 1 119 (2010)
PMID: 21081917

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-134725 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
T cell receptor alpha chain constant Chain: A
Molecule details ›
Chain: A
Length: 204 amino acids
Theoretical weight: 22.31 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01848 (Residues: 1-93; Coverage: 66%)
Gene names: TCRA, TRAC
Sequence domains: Domain of unknown function (DUF1968)
Structure domains: Immunoglobulins
T cell receptor beta constant 1 Chain: B
Molecule details ›
Chain: B
Length: 244 amino acids
Theoretical weight: 27.79 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01850 (Residues: 1-129; Coverage: 73%)
Gene name: TRBC1
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Enterotoxin type H Chain: C
Molecule details ›
Chain: C
Length: 217 amino acids
Theoretical weight: 25.18 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P0A0M0 (Residues: 25-241; Coverage: 100%)
Gene names: entH, seh
Sequence domains:
Structure domains:
HLA class II histocompatibility antigen, DR alpha chain Chain: D
Molecule details ›
Chain: D
Length: 182 amino acids
Theoretical weight: 21.16 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01903 (Residues: 26-207; Coverage: 80%)
Gene names: HLA-DRA, HLA-DRA1
Sequence domains:
Structure domains:
HLA class II histocompatibility antigen, DRB1 beta chain Chain: E
Molecule details ›
Chain: E
Length: 190 amino acids
Theoretical weight: 22.08 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01911 (Residues: 30-219; Coverage: 80%)
Gene name: HLA-DRB1
Sequence domains:
Structure domains:
Hemagglutinin HA1 chain Chain: F
Molecule details ›
Chain: F
Length: 13 amino acids
Theoretical weight: 1.51 KDa
Source organism: Influenza A virus
Expression system: Not provided
UniProt:
  • Canonical: Q03909 (Residues: 327-339; Coverage: 2%)
Gene name: HA

Ligands and Environments

2 bound ligands:
Ligand GOL 4 x GOL
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: C2
Unit cell:
a: 191.18Å b: 48.89Å c: 166.72Å
α: 90° β: 113.55° γ: 90°
R-values:
R R work R free
0.224 0.222 0.272
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli K-12
  • Not provided

Quick links

Citations

11 review citations

Basis of Virulence in Enterotoxin-Mediated Staphylococcal Food Poisoning.
Fisher et al. (2018)
10 more

8 mentions without citation

Conformational variation in structures of classical and non-classical MHCII proteins and functional implications.
Painter et al. (2012)
7 more