› 2xns

X-ray diffraction
3.41Å resolution

Crystal Structure Of Human G alpha i1 Bound To A Designed Helical Peptide Derived From The Goloco Motif Of RGS14

Released:
DOI: 10.2210/pdb2xns/pdb
PDB entry 2xns coloured by chain, front view.
PDB entry 2xns coloured by chain, side view.
PDB entry 2xns coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Computational design of the sequence and structure of a protein-binding peptide.
Sammond DW, Bosch DE, Butterfoss GL, Purbeck C, Machius M, Siderovski DP, Kuhlman B
J Am Chem Soc 133 4190-2 (2011)
PMID: 21388199

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128999 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Guanine nucleotide-binding protein G(i) subunit alpha-1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 327 amino acids
Theoretical weight: 37.37 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P63096 (Residues: 30-354; Coverage: 92%)
Gene name: GNAI1
Sequence domains: G-protein alpha subunit
Structure domains:
Regulator of G-protein signaling 14 Chains: C, D
Molecule details ›
Chains: C, D
Length: 40 amino acids
Theoretical weight: 4.7 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: O43566 (Residues: 497-517; Coverage: 4%)
Gene name: RGS14
Sequence domains: GoLoco motif

Ligands and Environments

3 bound ligands:
Ligand GDP 2 x GDP
Ligand SRT 2 x SRT
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: I23
Unit cell:
a: 265.261Å b: 265.261Å c: 265.261Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.223 0.244
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

5 review citations

Druggable protein-protein interactions--from hot spots to hot segments.
London et al. (2013)
4 more

6 mentions without citation

Strategies to control the binding mode of de novo designed protein interactions.
Der et al. (2013)
5 more