› 2xvl

X-ray diffraction
2.3Å resolution

crystal structure of alpha-xylosidase (GH31) from Cellvibrio japonicus in complex with Pentaerythritol propoxylate (5 4 PO OH)

Released:
DOI: 10.2210/pdb2xvl/pdb
PDB entry 2xvl coloured by chain, front view.
PDB entry 2xvl coloured by chain, side view.
PDB entry 2xvl coloured by chain, top view.
Source organism: Cellvibrio japonicus
Primary publication:
Structural and enzymatic characterization of a glycoside hydrolase family 31 α-xylosidase from Cellvibrio japonicus involved in xyloglucan saccharification.
Larsbrink J, Izumi A, Ibatullin FM, Nakhai A, Gilbert HJ, Davies GJ, Brumer H
Biochem J 436 567-80 (2011)
PMID: 21426303

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109882 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PA14 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 1020 amino acids
Theoretical weight: 115.2 KDa
Source organism: Cellvibrio japonicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B3PBD9 (Residues: 1-988; Coverage: 100%)
Gene names: CJA_2706, xyl31A
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand NI 3 x NI
Ligand SO4 3 x SO4
Ligand CL 7 x CL
Ligand PXN 1 x PXN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P6322
Unit cell:
a: 157.653Å b: 157.653Å c: 226.688Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.147 0.145 0.185
Expression system: Escherichia coli BL21(DE3)

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Citations

10 review citations

Polysaccharide Utilization Loci: Fueling Microbial Communities.
Grondin et al. (2017)
9 more