› 2xvo

X-ray diffraction
2.08Å resolution

SSO1725, a protein involved in the CRISPR/Cas pathway

Released:
DOI: 10.2210/pdb2xvo/pdb
PDB entry 2xvo coloured by chain, front view.
PDB entry 2xvo coloured by chain, side view.
PDB entry 2xvo coloured by chain, top view.
Source organism: Saccharolobus solfataricus
Primary publication:
Structure and mechanism of the CMR complex for CRISPR-mediated antiviral immunity.
Zhang J, Rouillon C, Kerou M, Reeks J, Brugger K, Graham S, Reimann J, Cannone G, Liu H, Albers SV, Naismith JH, Spagnolo L, White MF
Mol Cell 45 303-13 (2012)
PMID: 22227115

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189029 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative CRISPR system CMR subunit Cmr7 2 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 192 amino acids
Theoretical weight: 21.56 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q97XK8 (Residues: 1-190; Coverage: 100%)
Gene names: SSO1725, cmr7b
Structure domains: Jelly Rolls

Ligands and Environments

2 bound ligands:
Ligand SO4 2 x SO4
Ligand BME 3 x BME
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P212121
Unit cell:
a: 77.75Å b: 90.29Å c: 111.65Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.199 0.231
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

60 review citations

An updated evolutionary classification of CRISPR-Cas systems.
Makarova et al. (2015)
59 more

1 mention without citation

Structure and mechanism of the CMR complex for CRISPR-mediated antiviral immunity.
Zhang et al. (2012)